Coding

Part:BBa_K3519000

Designed by: Omkar Mohapatra   Group: iGEM20_IISER-Tirupati_India   (2020-10-07)
Revision as of 10:02, 7 October 2020 by Registry (Talk | contribs)

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sulX

This part only codes for sulX, sulfonamide monooxygenase. The genes sulX and sulR are a two-component flavin-dependent monooxygenase system present in Microbacterium sp. CJ77 and were first described by Kim et al., 2019. The expression of these genes in E. coli has been shown to degrade sulfonamides in the media.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 23
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 448
    Illegal AgeI site found at 576
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None