Part:BBa_K3610036
BAK1 ectodomain / mCherry N-terminal - for C. reinhardtii
This part is the cell surface receptor BAK1 from A. thaliana fused with a GFP. The two protein coding sequences are fused together with a 15 amino acid linker, coding for glycin and serine. As a promoter a truncated version of the ADH1 version was used as it confers lower expression levels. To mediate termination of transcription the ScENO2 terminator from S. cerevisiae was used.
BAK1/GFP is designed for the expression of a the full length plant receptor BAK1 from A. thaliana together with a fluorescent GFP tag in yeast. This allows checking for the localization and the level of expression of the BAK1 protein in the yeast cell. Being the full length cell surface protein, the BAK1 sequence has its own signal sequence from the orignal organism.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 93
Illegal NheI site found at 925 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 855
- 1000COMPATIBLE WITH RFC[1000]
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