Coding

Part:BBa_K3610017:Design

Designed by: Jonas Sebastian Trottmann   Group: iGEM20_UZurich   (2020-09-14)
Revision as of 09:30, 4 October 2020 by Jtrott (Talk | contribs) (→‎Design Notes)


SmallBit NanoLuc - codon optimized for C. reinhardtii


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codons with a frequency lower than 10% were considered rare and were replaced for optimal expression. As alternative codons, the ones with the highest frequencies were chosen unless it added unwanted restriction sites and was therefore incompatible with iGEM standards.

Source

The sequence was taken of Part:BBa_K3168003 from the iGEM registry and then codon optimized with SnapGene.


References

England, Christopher G.; Ehlerding, Emily B.; Cai, Weibo (2016): NanoLuc: A Small Luciferase Is Brightening Up the Field of Bioluminescence. In: Bioconjug Chem 27 (5), S. 1175–1187. DOI: 10.1021/acs.bioconjchem.6b00112.

Naya, Hugo; Romero, Héctor; Carels, Nicola; Zavala, Alejandro; Musto, Héctor (2001): Translational selection shapes codon usage in the GC-rich genome of Chlamydomonas reinhardtii. In: FEBS Letters 501 (2), S. 127–130. DOI: 10.1016/S0014-5793(01)02644-8.