Part:BBa_K3425018

iGEM Type IIS standard RBS template

This part is the constitutive promoter BBa_J23104 adopted to the iGEM Type IIS standard (RFC1000). The BBa_J23104 sequence is flanked by the Type IIS prefix and suffix which allows cloning into the Level 0 backbone (pSB1C00). BBa_J23100 is part of the Aderson promoter family and its relative strength is 0.72.

This part is the strong constitutive promoter BBa_J23100 adopted to the iGEM Type IIS standard (RFC1000). The BBa_J23100 sequence is flanked by the Type IIS prefix and suffix which allows cloning into the Level 0 backbone (pSB1C00). BBa_J23100 is part of the Anderson promoter family and its relative strength is 1. BBa_K3425017 can be ordered as two overlapping primers, see iGEM Type IIS guide.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI site found at 5
  Illegal SapI.rc site found at 39