Composite

Part:BBa_K3406005

Designed by: Xiaojie Zhou   Group: iGEM20_ZJUT_China_B   (2020-10-01)
Revision as of 11:47, 1 October 2020 by Sorrow-filter (Talk | contribs)

6xHis/Twin-strep-SUMO-CcaCas13b CcaCas13b is a member of Cas13 protein family.It has the common characteristic of Type VI RNA-targeting CRISPR–Cas system’s proteins, which means it could be actived by target RNA and could cleave bystander single strained RNAs. This feature could be used to target RNA and thus to detect RNA and to edit RNA. Besides,different Cas13 protein has different base cutting preference,and the preference of CcaCas13b is Poly U/UA/UC

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1436
    Illegal AgeI site found at 2288
  • 1000
    COMPATIBLE WITH RFC[1000]


Usage and Biology

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Cloning,expression and purification

References

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Categories
Parameters
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