Composite

Part:BBa_K3349007:Design

Designed by: Kristi Turton   Group: iGEM20_Lethbridge_HS   (2020-09-28)
Revision as of 17:30, 29 September 2020 by Kristiturton (Talk | contribs) (→‎Design Notes)


Pectin Lyase PelB


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 333
    Illegal BamHI site found at 1218
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part was codon optimized for use in E. coli . The his tag was placed on the C-terminus based on previous pectin lyase studies [4].

Source

The protein sequence was taken from the Uniprot server. The sequence ID from NCBI is WP_123064018.1.

References

1. Mei, Y., Chen, Y., Zhai, R., and Liu, Y. (2013) Cloning, purificationa nd biochemical properties of a thermostable pectinase from Bacillus haldurans M29.