Composite
Part:BBa_K3349006:Design
Designed by: Kristi Turton Group: iGEM20_Lethbridge_HS (2020-09-28)
Pectin Lyase Pnl
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The sequence was codon optimized for E. coli . The his tag was placed on the C-terminus as based by the cloning of other Pectin Lyase studies [1].
Source
Sequence was taken from the Uniprot database for synthesis. The sequence ID from NCBI is KAA8782864.1.