Part:BBa_K3570000
GGPP production enhancement in S. cerevisiae
One of the main goals of our project is to make S. Cerevisiae produce β-carotene. This construction is used in tight synergy with BBa_K3570001. Precisely, β-carotene derives from the mevalonate pathway as do limonene and geraniol (figure 1). We, therefore, decided to enhance the mevalonate pathway and the production of GGPP. According to Rabeharindranto et al. 2019, the enhancement of the mevalonate pathway can be achieved by overexpressing the gene HMG1 and CrtE. Our design differs from this publication in the choice of the promoter. The promoter TDH1 has been chosen instead of Gal1/10 because we planned to use this promoter for the regulation of other genes. We thus created the plasmids containing a truncated version of the HMG1 gene from S. Cerevisiae and the CrtE gene from X. Dendrorhous as on the figure 2.
Literature: Hery Rabeharindranto, Sara Castaño-Cerezo, Thomas Lautier, Luis F. Garcia-Alles, Christian Treitz, Andreas Tholey, Gilles Truan, 2019. Enzyme-fusion strategies for redirecting and improving carotenoid synthesis in S. cerevisiae. Metab Eng Commun. 2019 Jun; 8: e00086.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 1015
Illegal PstI site found at 50 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 683
Illegal NheI site found at 3586
Illegal NheI site found at 4491
Illegal NheI site found at 5834
Illegal PstI site found at 50 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1641
Illegal BglII site found at 3716
Illegal BglII site found at 5725
Illegal BglII site found at 5785
Illegal BamHI site found at 3376
Illegal XhoI site found at 705
Illegal XhoI site found at 3415
Illegal XhoI site found at 3547
Illegal XhoI site found at 4366 - 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 1015
Illegal PstI site found at 50 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 1015
Illegal PstI site found at 50
Illegal NgoMIV site found at 870 - 1000COMPATIBLE WITH RFC[1000]
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