Coding

Part:BBa_K3349003:Design

Designed by: Kristi Turton   Group: iGEM20_Lethbridge_HS   (2020-09-23)
Revision as of 15:54, 23 September 2020 by Registry (Talk | contribs)

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Pectin Lyase Pnl-GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence was codon optimized for E. coli . The his tag was placed on the C-terminus.


Source

The GFP protein was taken from the uniprot database (P42212). The pectin lyase was also taken from the uniprot database and the sequence ID from NCBI is KAA8782864.1.

References