Coding
Part:BBa_K3447000:Design
Designed by: Jiahao Yu, Xinyue Zhang, Yu Ma Group: iGEM20_Jilin_China (2020-09-03)
luxI, an autoinducer synthetase for AHL
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
There is a repetitive sequence between cvi and cvaC gene, and other than that there is no need to pay attention.
Source
We will use cvaAB to express channel proteins for the transport of ColV out of the cell membrane.
This gene is derived from Escherichia coli (strain: A2363), we get the genomic sequence by NCBI.
https://www.ncbi.nlm.nih.gov/nuccore/NC_007675.1?report=genbank&from=155164&to=156075
https://www.ncbi.nlm.nih.gov/nuccore/NC_007675.1?report=genbank&from=156050&to=158164
We ordered the part DNA from a synthesis company.