Coding
Part:BBa_K3447000:Design
Designed by: Jiahao Yu, Xinyue Zhang, Yu Ma Group: iGEM20_Jilin_China (2020-09-03)
luxI, an autoinducer synthetase for AHL
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
abaaba
Source
We will use cvaAB to express channel proteins for the transport of ColV out of the cell membrane.
This gene is derived from Escherichia coli (strain: A2363), we get the genomic sequence by NCBI.
https://www.ncbi.nlm.nih.gov/nuccore/NC_007675.1?report=genbank&from=155164&to=156075
https://www.ncbi.nlm.nih.gov/nuccore/NC_007675.1?report=genbank&from=156050&to=158164
We ordered the part DNA from a synthesis company.