Part:BBa_K118024

dxs+LIMS1+appY

dxs, from Escherichia coli JM109, encodes 1-deoxyxylulose-5-phosphate synthase, thiamine-requiring, which catalyses the first step in the biosynthesis of terpenoids, and appY, also from Escherichia coli JM109, encodes a transcriptional regulator related to anaerobic energy metabolism. Overexpression of dxs and appYhas been reported to increase yields of carotenoids and other terpenoids (Kang, M.J., Lee, Y.M., Yoon, S.H., Kim, J.H., Ock, S.W., Jung, K.H., Shin, Y.C., Keasling, J.D., and Kim, S.W. 2005. Identification of genes affecting lycopene accumulation in Escherichia coli using a shot-gun method. Biotechnology and Bioengineering 91, 636-642). LIMS1, from Citrus limon, encodes limonene synthase, which produces (+)-limonene from farnesyl diphosphate (an intermediate in the mevalonate pathway)(Lucker, J., El Tamer, M.K., Schwab, W., Verstappen, F.W.A., van der Plas, L.H.W., Bouwmeester, H.J., and Verhoeven, H.H. 2002. Monoterpene biosynthesis in lemon (Citrus limon). European Journal of Biochemistry 269, 3160-3171). (+)-Limonene is a component of lemon scent. (All three genes are individually preceded by a ribosome binding site.)

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 746
  Illegal SapI.rc site found at 2006