Regulatory
Tat PROM

Part:BBa_K895000

Designed by: Frank Sargent   Group: iGEM12_Dundee   (2012-09-21)
Revision as of 20:06, 21 September 2012 by Franksargent (Talk | contribs)

Eco_Ptat2

The constitutive promoter region (103 bp) from the Escherichia coli K-12 tatABCD (twin-arginine translocase) operon. This is a constitutive promoter that gives good levels of expression in E. coli, but is not inducible or repressible. The promoter region is cloned as an EcoRI / PstI fragment into pSB1C3 following Biobrick Standard RFC[10]. This is a corrected part that replaces the BBa_K562000 biobrick.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 98
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None