Composite

Part:BBa_K3458004:Design

Designed by: Jiahua Zou   Group: iGEM20_GDSYZX   (2020-08-16)
Revision as of 03:34, 17 August 2020 by Kawah (Talk | contribs) (→‎Design Notes)


GluD-1 Promoter + HQT


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 225


Design Notes

The goal of our team is to specifically express HQT, a key enzyme for chlorogenic acid synthesis in the endosperm of rice. The promoter GluD-1 cannot drive the HQT express in Oryza sativa L. protoplasts. In order to test our expression system in protoplasts, we designed a 35s promoter + HQT (BBa_K3458004) composite element for comparison with this part.

Source

A

References