Coding
Part:BBa_K3458001:Design
Designed by: Jiahua Zou Group: iGEM20_GDSYZX (2020-08-14)
HQT(Hydroxycinnamoyl-CoA Quinate Hydroxycinnamoyl Transferase)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
- We synthesized this DNA sample through TIANYI HUIYUAN Company.
In order to have a better expression in Oryza sativa L., we have carried out codon optimization according to the codon preference of Oryza sativa L..
- In order to meet the assembly requirements, we removed the illegal restriction site.
- In order to express HQT in Oryza sativa L. and conduct further tests, we also designed two composite originals, GluD-1::HQT (BBa_K3458004) and 35S::HQT (BBa_K3458003).
Source
The HQT gene we used comes from the genome of Lonicera japonica (GeneBank:[1]). We removed the restriction site incompatible with the biobrick assembly standard and optimized the codon according to the codon preference of Oryza sativa L..The HQT was synthesized in TIANYI HUIYUAN Company.