Part:BBa_K3600002

YCF1 promoter part plasmid

This part contains the promoter of the gene YCF1 from S. Cerevisiae.

Usage and Biology

• activator: Yap1p
• YCF1 gene encodes the Yeast cadmium factor 1 ³.
• The YCF1 gene is required for yeast cells to better resist exposure to cadmium.⁴
• The promoter YCF1 has been chosen in order to express fluorescent protein [ref] when the S.cervisiae is exposed to toxic effects.
• The binding site for Yap1p, in the promoter YCF1, is TTACTAA.(ref)
• The yeast toolkit for modular assembly ² was used to assemble transcriptional units. This promoter was used as a part of type II.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 935
  Illegal EcoRI site found at 1665
  Illegal XbaI site found at 944
  Illegal XbaI site found at 1842
  Illegal SpeI site found at 2810
  Illegal PstI site found at 2819
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 935
  Illegal EcoRI site found at 1665
  Illegal SpeI site found at 2810
  Illegal PstI site found at 2819
  Illegal NotI site found at 923
  Illegal NotI site found at 2829
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal suffix found in sequence at 2412
  Illegal EcoRI site found at 935
  Illegal EcoRI site found at 1665
  Illegal BglII site found at 1280
  Illegal BglII site found at 2283
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 935
  Illegal EcoRI site found at 1665
  Illegal XbaI site found at 944
  Illegal XbaI site found at 1842
  Illegal SpeI site found at 2810
  Illegal PstI site found at 2819
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 935
  Illegal EcoRI site found at 1665
  Illegal XbaI site found at 944
  Illegal XbaI site found at 1842
  Illegal SpeI site found at 2810
  Illegal PstI site found at 2819
  Illegal NgoMIV site found at 2767
  
• 1000
  COMPATIBLE WITH RFC[1000]

References

[1]

[3] Bhuiyan, M. S. U. et al. Overexpression of a yeast cadmium factor 1 (YCF1) enhances heavy metal tolerance and accumulation in Brassica juncea. Plant Cell Tiss Organ Cult 105, 85–91 (2011).

[2] Lee, M. E., DeLoache, W. C., Cervantes, B. & Dueber, J. E. A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly. ACS Synth. Biol. 4, 975–986 (2015).

[4] Li, Z.-S., Szczypka, M., Lu, Y.-P., Thiele, D. J. & Rea, P. A. The Yeast Cadmium Factor Protein (YCF1) Is a Vacuolar Glutathione S-Conjugate Pump. J. Biol. Chem. 271, 6509–6517 (1996).