Plasmid
GSH1pr

Part:BBa_K3600003:Design

Designed by: Sven Spoerri, Yasmine Koubaa   Group: iGEM20_EPFL   (2020-06-14)
Revision as of 19:26, 14 June 2020 by Sspoerri (Talk | contribs) (→‎References)


GSH1 promoter part plasmid


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 975
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 563
    Illegal BsaI site found at 972
    Illegal BsaI.rc site found at 1084


Design Notes

The sequence of 500bp preceding the ORF of GSH1 was retrieved from the yeast genome database. No modifications were applied. The presence of the GSHI yAP-1 response element (YRE) was confirmed by sequence analysis.

Source

Promoter of the gamma-glutamylcysteine synthetase (YJL101C) from the genome of S. Cerevisiae.

References

[1] Wu, A. L. & Moye-Rowley, W. S. GSH1, which encodes gamma-glutamylcysteine synthetase, is a target gene for yAP-1 transcriptional regulation. Molecular and Cellular Biology 14, 5832–5839 (1994).