Plasmid
TRX2pr

Part:BBa_K3600001:Design

Designed by: Sven Spoerri, Yasmine Koubaa   Group: iGEM20_EPFL   (2020-06-11)
Revision as of 15:15, 11 June 2020 by Sspoerri (Talk | contribs) (→‎Design Notes)


TRX2 promoter part plasmid


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 568
    Illegal BsaI.rc site found at 1089


Design Notes

The sequence of 500bp preceding the ORF of TRX2 was retrieved from the yeast genome database. The presence of two Yap1p binding sites was confirmed by sequence analysis. Yap1p is a transcription factor necessary for oxidative stress tolerance.

Source

Promoter of the cytoplasmic thioredoxin isoenzyme (YGR209C) from the genome of S. Cerevisiae.

References