Part:BBa_K3515007:Design
Troponin C, a calcium binding protein with cysteine modification(s) to bind to a biosensor and FRET
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1093
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Added a cysteine for immobilization (K93C) opposite to the active site region for linker arm immobilization and removed a cysteine previously existing (C101A). Amino acid substitution considerations were made using an intensive BLAST search to ensure conservation. N109D, D111N, N145D, and D147N modifications were made based on previous evidence of these substitutions permitting selective calcium binding in the physiological range and removing the ability of magnesium to bind competitively.
Source
The source of this part is its sequence retrieved from the European Nucleotide Archive (M19027.1) along with our own modifications.
References
Mank, M., Reiff, D.F., Heim, N., Friedrich, M.W., Borst, A. and Griesbeck, O., 2006. A FRET-based calcium biosensor with fast signal kinetics and high fluorescence change. Biophysical journal, 90(5), pp.1790-1796