Tag
OmpA

Part:BBa_K103006

Designed by: Michael Lower   Group: iGEM08_Warsaw   (2008-09-14)
Revision as of 17:33, 24 October 2008 by Keton (Talk | contribs)

OmpA outer membrane protein A fused to linker; displays proteins on cell surface

One of our basic bricks used to create fusions attached to outer membrane

Usage and Biology

  • OmpA is outer membrane protein from E. coli [http://www.ncbi.nlm.nih.gov/pubmed/17559395?ordinalpos=1&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSum]
  • Proteins fused to Ompa-link are presented on cell's surface
  • Unstructuralized linker (Gly Ser Gly) allows proper folding of both fusion partners
  • In our project Ompa-link is used as outer membrane anchor for our selection system
  • This brick contains our nonstandard restriction sites (NdeI and SacI) that allow 'scarless' cloning and easy creation of translation fusions

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//proteindomain/localization
Parameters
None