Coding
Part:BBa_K3515009:Design
Designed by: Kody Klupt, Sono Khan Group: iGEM20_Queens_Canada (2020-05-25)
Glucose binding protein with cysteine modification(s) to bind to a biosensor and FRET to monitor glu
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Added a cysteine for immobilization (D190C) opposite to the active site region for linker arm immobilization. Amino acid substitution considerations were made using an intensive BLAST search to ensure conservation.
Source
The source of this part is its sequence retrieved from the European Nucleotide Archive (M59444.1) along with our own modifications.