Regulatory

Part:BBa_J100534:Design

Designed by: Cathleen Krabak, Lindsey Voelker, Kate Kilpatrick, Eboni Thomas   Group: Campbell M Lab   (2020-02-09)
Revision as of 03:58, 10 February 2020 by Registry (Talk | contribs)

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Constitutive RFP Promoter in E. coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 35
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We decided not to order the AHL chemical because there is no lux cassette in the E. coli cultures that we will use (not possible for the lux gene to be expressed). Therefore, we are allowing it to function as a constitutive promoter.


Source

This promoter sequence came from an article published on NCBI: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4444344/


References