Composite

Part:BBa_K3002209

Designed by: Dorothée Klein   Group: iGEM19_TU_Kaiserslautern   (2019-10-21)
Revision as of 01:31, 22 October 2019 by MSchlosser (Talk | contribs)


L2 spectinomycin resistance + cCA_Mut-PETase

This composite part contains a spectinomycin resistance (BBa_K3002102) and the Mutant PETase (BBa_K3002106) fused with an HA-tag for easy detection via HA-antibody and with the secretion signal cCA.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2401
    Illegal PstI site found at 3485
    Illegal PstI site found at 4458
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2401
    Illegal NheI site found at 2665
    Illegal PstI site found at 3485
    Illegal PstI site found at 4458
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2401
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2401
    Illegal PstI site found at 3485
    Illegal PstI site found at 4458
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2401
    Illegal PstI site found at 3485
    Illegal PstI site found at 4458
    Illegal NgoMIV site found at 1401
    Illegal NgoMIV site found at 1584
    Illegal NgoMIV site found at 1694
    Illegal NgoMIV site found at 3220
    Illegal NgoMIV site found at 3247
    Illegal NgoMIV site found at 4898
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None