Composite
Part:BBa_K3002123
Designed by: Marlene Schlosser Group: iGEM19_TU_Kaiserslautern (2019-10-21)
Revision as of 01:08, 22 October 2019 by MSchlosser (Talk | contribs)
Level 1 MHETase + cCA + sp20-His
This composite part contains the PAR-promoter (BBa_K3002027) in combination with the RPL23-Terminator (BBa_K3002006), the SP20-His-tag (BBa_K3002018), the secretion signal cCA (BBa_K3002007) and the coding sequence of the MHETase (BBa_K3002037) plus the MoClo connectors for positions B1-B2 (BBa_K3002302), B2-B3 (BBa_K3002303), B4-B5 (BBa_K3002304) and B5-B6 (BBa_K3002305).
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 1327
Illegal PstI site found at 1651
Illegal PstI site found at 1994
Illegal PstI site found at 2804 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 249
Illegal PstI site found at 1327
Illegal PstI site found at 1651
Illegal PstI site found at 1994
Illegal PstI site found at 2804
Illegal NotI site found at 1662 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2572
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 1327
Illegal PstI site found at 1651
Illegal PstI site found at 1994
Illegal PstI site found at 2804 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 1327
Illegal PstI site found at 1651
Illegal PstI site found at 1994
Illegal PstI site found at 2804
Illegal NgoMIV site found at 1260
Illegal NgoMIV site found at 1721
Illegal NgoMIV site found at 1751
Illegal NgoMIV site found at 2066
Illegal NgoMIV site found at 2084
Illegal NgoMIV site found at 2120
Illegal NgoMIV site found at 2763 - 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |