Composite

Part:BBa_K3002233

Designed by: Marlene Schlosser   Group: iGEM19_TU_Kaiserslautern   (2019-10-21)
Revision as of 02:24, 22 October 2019 by MSchlosser (Talk | contribs)


L2 spectinomycin resistance + MutateM_HA

This composite part contains a spectinomycin resistance (BBa_K3002102) and the Mutate M from iGEM16_TJUSLS_China (BBa_K3002101) fused with an HA-tag for easy detection via HA-antibody.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2401
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2401
    Illegal NheI site found at 2665
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2401
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2401
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2401
    Illegal NgoMIV site found at 1401
    Illegal NgoMIV site found at 1584
    Illegal NgoMIV site found at 1694
    Illegal NgoMIV site found at 4138
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None