Composite

Part:BBa_K3002210

Designed by: Dorothée Klein   Group: iGEM19_TU_Kaiserslautern   (2019-10-21)
Revision as of 01:33, 22 October 2019 by MSchlosser (Talk | contribs)


L2 spectinomycin resistance + ARS_Mut-PETase + cCA_MHETase

This composite part contains a spectinomycin resistance (BBa_K3002102), the mutant PETase with the secretion signal ARS (BBa_K3002108) and the MHETase with the cCA (BBa_K3002113), both fused with an HA-tag for easy detection via HA-antibody.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2401
    Illegal EcoRI site found at 5303
    Illegal PstI site found at 3491
    Illegal PstI site found at 4464
    Illegal PstI site found at 6645
    Illegal PstI site found at 6969
    Illegal PstI site found at 7312
    Illegal PstI site found at 8122
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2401
    Illegal EcoRI site found at 5303
    Illegal NheI site found at 2665
    Illegal NheI site found at 5567
    Illegal PstI site found at 3491
    Illegal PstI site found at 4464
    Illegal PstI site found at 6645
    Illegal PstI site found at 6969
    Illegal PstI site found at 7312
    Illegal PstI site found at 8122
    Illegal NotI site found at 6980
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2401
    Illegal EcoRI site found at 5303
    Illegal BglII site found at 7890
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2401
    Illegal EcoRI site found at 5303
    Illegal PstI site found at 3491
    Illegal PstI site found at 4464
    Illegal PstI site found at 6645
    Illegal PstI site found at 6969
    Illegal PstI site found at 7312
    Illegal PstI site found at 8122
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2401
    Illegal EcoRI site found at 5303
    Illegal PstI site found at 3491
    Illegal PstI site found at 4464
    Illegal PstI site found at 6645
    Illegal PstI site found at 6969
    Illegal PstI site found at 7312
    Illegal PstI site found at 8122
    Illegal NgoMIV site found at 1401
    Illegal NgoMIV site found at 1584
    Illegal NgoMIV site found at 1694
    Illegal NgoMIV site found at 3226
    Illegal NgoMIV site found at 3253
    Illegal NgoMIV site found at 4904
    Illegal NgoMIV site found at 6578
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None