Tag

Part:BBa_K3002017

Designed by: Dorothée Klein   Group: iGEM19_TU_Kaiserslautern   (2019-10-13)
Revision as of 13:19, 16 October 2019 by Kleind (Talk | contribs)


3xHA tag for Chlamydomonas reinhardtii (Phytobrick)

This basic part contains the sequence of the 3xHA-tag (B5) and was built as a part of the Kaiser Collection. Combined with a promoter (e.g. BBa_K3002016 (PSAD promoter (A1-B2)) or BBa_K3002027 (PAR promoter (A1-B2))), a CDS and terminator (e.g. BBa_K3002006 (RPL23 terminator)) your target protein can be detected via HA-antibody.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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