Protein_Domain

Part:BBa_K3002010

Designed by: Dorothée Klein   Group: iGEM19_TU_Kaiserslautern   (2019-10-16)
Revision as of 03:59, 22 October 2019 by Bronja (Talk | contribs)


Sp20 HA tag for Chlamydomonas reinhardtii (Phytobrick)

This basic part contains the sequence of the sp20 3xHA-tag (B5) and was built as a part of the Kaiser Collection. Combined with a secretion signal of the Kaiser Collection like BBa_K3002007 (cCA (B2)), BBa_K3002008 (GLE (B2)) or BBa_K3002009 (ARS (B2)), an appropriate promoter (BBa_K3002001 (PSAD promoter (A1-B1)) or BBa_K3002031 (PAR promoter (A1-B1))) and terminator (e.g. BBa_K3002006 (RPL23 terminator)) your target protein is secreted efficiently and can be detected via HA-antibody (Ramos-Martinez, 2017).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None