Intermediate

Part:BBa_K3032128

Designed by: Gytis Druteika   Group: iGEM19_Vilnius-Lithuania   (2019-10-16)
Revision as of 23:55, 16 October 2019 by Gytisdruteika (Talk | contribs)


Light-regulated plasmid copy number device intermediate (0.86)

This is an intermediate construct to the light-regulated plasmid copy number system. It consists of an Anderson promoter (BBa_J23102) with light-activated protein EL222 binding sequence inserted between -35 and -10 promoter regions and ColE1 RNA I gene (without promoter).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 31
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None