Regulatory

Part:BBa_J100301

Designed by: Hannah Sinks   Group: Campbell M Lab   (2016-09-08)
Revision as of 14:23, 15 September 2016 by Hasinks (Talk | contribs)


ompW Promoter

The promoter ompW is regulated by the inhibitor protein ArcA. In the presence of 4mM hydrogen peroxide, ArcA is activated and, therefore, ompW is inhibited. For our project, we will use a 56-base pair long sequence of DNA that we will place into a receiving plasmid using BsaI sticky ends. We will then use bacterial transformation to determine the functionality of our promoter sequence.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None