Composite

Part:BBa_K2916038:Design

Designed by: Konstantinos Ragios   Group: iGEM19_EPFL   (2019-09-25)
Revision as of 19:27, 25 September 2019 by Konstantinos (Talk | contribs) (→‎Design Notes)


Expression of EF-Tu in E.coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1292
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 389
    Illegal AgeI site found at 794
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 539


Design Notes

The protein was expressed in pQE60 in M15 E.coli strain.

Source

Sebastian Maerkl Lab at EPFL, Switzerland.

http://www.addgene.org/124127/

References

Cell-free translation reconstituted with purified components. Shimizu Y, Inoue A, Tomari Y, Suzuki T, Yokogawa T, Nishikawa K, Ueda T. Nat Biotechnol. 2001 Aug;19(8):751-5. doi: 10.1038/90802. 10.1038/90802 PubMed 11479568

Lavickova, Barbora, and Sebastian J. Maerkl. A Simple, Robust, and Low-Cost Method to Produce the PURE Cell - Free System. preprint, Synthetic Biology, 18 Sept. 2018. DOI.org (Crossref), doi:10.1101/420570.