Regulatory
Pcel

Part:BBa_K3093001

Designed by: Miao Zongjie   Group: iGEM19_ECUST_China   (2019-10-16)
Revision as of 02:32, 22 October 2019 by Still Water (Talk | contribs)


chbB promoter

ChbB promotor is the promotor of chitobiose operon. It has the NagC, ChbR and CAP binding sites and can be activated by N -acetylglucosamine disaccharide, chitobiose. [More information is available at EcoCyc: EG10143].

The chbB promotor was obtained from E.coli MG1655 through PCR. And we remove the strong NagC binding site.


References

[1]Plumbridge J, Pellegrini O. Expression of the chitobiose operon of Escherichia coli is regulated by three transcription factors: NagC, ChbR and CAP. Mol Microbiol. 2004 Apr;52(2):437-49. PubMed PMID: 15066032.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None