Composite

Part:BBa_K2916073:Design

Designed by: Konstantinos Ragios   Group: iGEM19_EPFL   (2019-09-30)
Revision as of 22:29, 21 October 2019 by Konstantinos (Talk | contribs) (→‎Source)


Toehold switch for Bois Noir 2.1 with superfolder GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 96
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 141


Source

The toehold has incorporated as an overhand on the primer designed to amplify sfGFP and the composite part was produced with PCR.

References

Green, Alexander A., et al. “Toehold Switches: De-Novo-Designed Regulators of Gene Expression.” Cell, vol. 159, no. 4, Nov. 2014, pp. 925–39. DOI.org (Crossref), doi:10.1016/j.cell.2014.10.002.