Regulatory

Part:BBa_K102912

Designed by: Wayne Materi   Group: iGEM08_Alberta_NINT   (2008-10-26)
Revision as of 01:42, 27 October 2008 by Registry (Talk | contribs)

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TA12 plus pause sequence

This design incorporate stem bulges and YUNR sequence in the loop (5' UUGG) as per Isaacs et al, 2004 to improve RNA stability and susceptibility to anti-sense induction. In addition a "pause" sequence (5' atgtgtgct) is added at the 3' end of the stem.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 14
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None