Composite

Part:BBa_K3002222

Designed by: Marlene Schlosser   Group: iGEM19_TU_Kaiserslautern   (2019-10-21)
Revision as of 19:39, 21 October 2019 by Registry (Talk | contribs)

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L2 spectinomycin resistance + ARS_Mut-PETase_SP20His

This composite part contains a spectinomycin resistance (BBa_K3002102) and the mutant PETase with the secretion signal ARS (BBa_K3002121) fused with an SP20His-tag for easy purification via Ni-NTA column and enhanced secretion.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2401
    Illegal PstI site found at 3491
    Illegal PstI site found at 4464
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2401
    Illegal NheI site found at 2665
    Illegal PstI site found at 3491
    Illegal PstI site found at 4464
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2401
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2401
    Illegal PstI site found at 3491
    Illegal PstI site found at 4464
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2401
    Illegal PstI site found at 3491
    Illegal PstI site found at 4464
    Illegal NgoMIV site found at 1401
    Illegal NgoMIV site found at 1584
    Illegal NgoMIV site found at 1694
    Illegal NgoMIV site found at 3226
    Illegal NgoMIV site found at 3253
    Illegal NgoMIV site found at 4955
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None