Composite

Part:BBa_K3002202

Designed by: Dorothée Klein   Group: iGEM19_TU_Kaiserslautern   (2019-10-21)
Revision as of 19:12, 21 October 2019 by Registry (Talk | contribs)

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L2 spectinomycin resistance + cCA_Mut-PETase + cCA_MHETase

This composite part contains a spectinomycin resistance (BBa_K3002102), the Mutant PETase (BBa_K3002106) and the wildtype MHETase (BBa_K3002113), both are fused with an HA-tag for easy detection via HA-antibody and with the secretion signal cCA.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2401
    Illegal EcoRI site found at 5297
    Illegal PstI site found at 3485
    Illegal PstI site found at 4458
    Illegal PstI site found at 6639
    Illegal PstI site found at 6963
    Illegal PstI site found at 7306
    Illegal PstI site found at 8116
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2401
    Illegal EcoRI site found at 5297
    Illegal NheI site found at 2665
    Illegal NheI site found at 5561
    Illegal PstI site found at 3485
    Illegal PstI site found at 4458
    Illegal PstI site found at 6639
    Illegal PstI site found at 6963
    Illegal PstI site found at 7306
    Illegal PstI site found at 8116
    Illegal NotI site found at 6974
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2401
    Illegal EcoRI site found at 5297
    Illegal BglII site found at 7884
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2401
    Illegal EcoRI site found at 5297
    Illegal PstI site found at 3485
    Illegal PstI site found at 4458
    Illegal PstI site found at 6639
    Illegal PstI site found at 6963
    Illegal PstI site found at 7306
    Illegal PstI site found at 8116
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2401
    Illegal EcoRI site found at 5297
    Illegal PstI site found at 3485
    Illegal PstI site found at 4458
    Illegal PstI site found at 6639
    Illegal PstI site found at 6963
    Illegal PstI site found at 7306
    Illegal PstI site found at 8116
    Illegal NgoMIV site found at 1401
    Illegal NgoMIV site found at 1584
    Illegal NgoMIV site found at 1694
    Illegal NgoMIV site found at 3220
    Illegal NgoMIV site found at 3247
    Illegal NgoMIV site found at 4898
    Illegal NgoMIV site found at 6572
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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