Device
Part:BBa_K3292001
Designed by: Abril Diosdado Hernandez, Gerardo David Diaz Renovado Group: iGEM19_Tec-Monterrey (2019-10-16)
T7-LacO Promoter + strong RBS + sialidase + sfGFP + 6x his-tag + double terminator
This device uses Bba_K2406020, Bba_J34801 and Bba_0010 to regulate the expression of a super folding GFP (Bba_I746916). The main objective of creating this device is to facilitate the secretion and characterization of any protein intracellularly produced after adding its nucleotide sequence next to the sfGFP.
The characterization of this part included a Bradford assay to quantify the amount of protein present in the medium for each of the proposed constructs. The conditions for the assay are listed below.
The other characterization assay was a fluorescent measurement, bacteria were growth at 30ºC for 3 hrs. Then
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 721
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1986
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 646
Illegal BsaI.rc site found at 510
Illegal BsaI.rc site found at 1647
Illegal SapI.rc site found at 2055
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Categories
Parameters
None |