Part:BBa_K2992008

Thiolase gene thl from C. acetobutylicum.

The thiolase gene encodes an acetyl-coenzyme A acetyltransferases from C. acetobutylicum.

Usage and Biology

Thiolases are core metabolic enzymes involved in the melavonate pathway responsible for the conversion of acetyl-CoA to acetoacetyl-CoA. Results from our genome scale modeling suggested that exogenous thiolase was required for the production of acetone in our chosen volatile reporter organism C. sporogenes. Thiolase was therefore included in our acetone production pathway constructs in order to a volatile reporter for the prediction of botulinum toxin production following food manufacturing processes.

Characterisation

This basic part was used as a composite in BBa_K2992028, BBa_K2992029, BBa_K2992030, BBa_K2992034, BBa_K2992035, BBa_K2992036. This was then characterised using fluorescent assay, the results of which are shown below. See the results page for more information.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal XbaI site found at 355
  
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal XbaI site found at 355
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal XbaI site found at 355
  
• 1000
  COMPATIBLE WITH RFC[1000]

References

Dennis P. Wiesenborn, E. (1988). Thiolase from Clostridium acetobutylicum ATCC 824 and Its Role in the Synthesis of Acids and Solvents. Applied and Environmental Microbiology, [online] 54(11), p.2717. Available at: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC204361/ [Accessed 21 Oct. 2019].