Composite

Part:BBa_K3002112

Designed by: Marlene Schlosser   Group: iGEM19_TU_Kaiserslautern   (2019-10-21)
Revision as of 15:38, 21 October 2019 by Registry (Talk | contribs)

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Level 1 MHETase + HA

This composite part contains the PAR-promoter of the team iGEM17_NU_Kazakhstan (BBa_K2516001) in combination with the RPL23-Terminator (BBa_K3002006), the HA-tag (BBa_K3002017) and the coding sequence of the Wildtype-MHETase (BBa_K3002037) plus the MoClo connectors for positions B2-B3 (BBa_K3002303), B4-B5 (BBa_K3002304) and B5-B6 (BBa_K3002305).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1400
    Illegal PstI site found at 1724
    Illegal PstI site found at 2067
    Illegal PstI site found at 2877
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 264
    Illegal PstI site found at 1400
    Illegal PstI site found at 1724
    Illegal PstI site found at 2067
    Illegal PstI site found at 2877
    Illegal NotI site found at 1735
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2645
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1400
    Illegal PstI site found at 1724
    Illegal PstI site found at 2067
    Illegal PstI site found at 2877
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1400
    Illegal PstI site found at 1724
    Illegal PstI site found at 2067
    Illegal PstI site found at 2877
    Illegal NgoMIV site found at 1333
    Illegal NgoMIV site found at 1794
    Illegal NgoMIV site found at 1824
    Illegal NgoMIV site found at 2139
    Illegal NgoMIV site found at 2157
    Illegal NgoMIV site found at 2193
    Illegal NgoMIV site found at 2836
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None