Regulatory

Part:BBa_K3189001

Designed by: Jehoshua Sharma, Nathanael Wilms, Connor Gianetto-Hill   Group: iGEM19_Guelph   (2019-10-16)
Revision as of 00:31, 22 October 2019 by Cgianetto (Talk | contribs)


Phage Lambda-TetO

A modified phage lambda PL promoter with tet operator sites. This part functions as a promoter that allows for tetracycline-based transcriptional activation


The flasks in Figure 1 shows 50 mL E.coli cells transformed with gfp under the control of BBa_K3189001. On the plasmid containing BBa_K3189001 is the repressor, BBa_K3189004, needed for proper function of the promoter. Tetracycline was used to induce the system, resulting in GFP production and fluorescence is seen using a UV wand.
T--Guelph--tetO_characterization.JPG
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T--Guelph--GPRexpression.PNG
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800px-T--Guelph--96-well_plate.jpeg
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216px-T--Guelph--pTA-tetnotet.jpg
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207px-T--Guelph--pTAtetnotetspun.jpg
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Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None