Coding
Part:BBa_K3113005
Designed by: Theresa Keil Group: iGEM19_Munich (2019-09-04)
Revision as of 13:09, 21 October 2019 by Theresakeil (Talk | contribs)
FLuc
This DNA sequence codes for the firefly luciferase. The firefly luciferase (FLuc) is a light-emitting enzyme. This sequence has its origin in the organism Photinus pyralis.
Usage
The readout of ALiVE is based on RNA. We used FLuc as message loaded into the vesicles. This had the advantage, that we could test our vesicles for passive transfection. The cells treated with our vesicle could be examined for passive transfection via a FLuc assay.
Biology
FLuc is an oxidative enzyme, producing bioluminescence in the presence of the substrate luciferin and oxygen.
Characterization
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Usage
[edit]
Categories
Parameters
None |