Coding

Part:BBa_K3113005

Designed by: Theresa Keil   Group: iGEM19_Munich   (2019-09-04)
Revision as of 13:09, 21 October 2019 by Theresakeil (Talk | contribs)


FLuc

This DNA sequence codes for the firefly luciferase. The firefly luciferase (FLuc) is a light-emitting enzyme. This sequence has its origin in the organism Photinus pyralis.

Usage

The readout of ALiVE is based on RNA. We used FLuc as message loaded into the vesicles. This had the advantage, that we could test our vesicles for passive transfection. The cells treated with our vesicle could be examined for passive transfection via a FLuc assay.

Biology

FLuc is an oxidative enzyme, producing bioluminescence in the presence of the substrate luciferin and oxygen.

Characterization

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Usage


[edit]
Categories
Parameters
None