RNA

Part:BBa_K3015000

Designed by: Valentina Stuchlik   Group: iGEM19_BOKU-Vienna   (2019-10-13)
Revision as of 00:18, 22 October 2019 by Kamis (Talk | contribs)


Mycolactone-Riboswitch

Riboswitch specific for Mycolactone. Consists of an Aptamer-sequence, a spacer region, a terminator and a U-Stretch. In the absence of Mycolactone the terminator-sequence will bind to the aptamer-sequence and form a hairpin, which will cause the RNA-Polymerase to dissociate from the DNA-strand. In the presence of Mycolactone it will bind to the Aptamer-sequence and loosen the hairpin structure and the RNA-Polymerase can transcribe.
T--BOKU-Vienna--K3015001mycolactone.png
Figure 1: Secondary structure of Mycolactone aptamer with intrinsic termination

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None