Part:BBa_K3113008

HiBiT

HiBiT is a peptide tag used for antibody-free endogenous protein detection. It is 11 amino acids long and binds with high affinity to another larger subunit called LgBiT. The bound complex has luciferase activity. Promega has all rights to this sequence.

Usage

HiBiT allows you to quantify the amount of tagged protein relatively quick via a luminescence assay. We are using this part fused to the vesicle forming proteins to measure the export rate of vesicles and the efficiency of purification.

Biology

“The Nano-Glo® HiBiT Lytic Detection System quantifies cellular protein in minutes with high sensitivity and a broad dynamic range using a simple add-mix-read assay format.” The HiBiT peptide is an 11 amino acid long tag that can be added to the protein of interest. The tagged protein can be measured with the Nano-Glo® HiBiT Detection Reagent which contains the LbBiT. Together HiBiT and LgBiT form a functioning luminescent NanoBiT® enzyme.

Characterization

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]