Reporter

Part:BBa_K3267001

Designed by: Yuma Kanazawa   Group: iGEM19_NYU_Shanghai   (2019-10-15)
Revision as of 23:01, 21 October 2019 by Yumakk (Talk | contribs) (→‎Biology and Usage)


Quorum-Sensing Receiver Construct

Transformed cells with this construct respond to AHL-dependent quorum sensing and GFP would be expressed by the activated transcription factor protein LuxR which binds to the bidirectional promoter region (pLuxR/pLuxI).

Biology and Usage

This Receiver part contains the V. fischeri-intrinsic bidirectional promoter region, the LuxRS regulon. The high concentration of Acyl-homoserine lactose (AHL) binds to the LuxR protein to activate and it binds to pLuxR/pLuxS promoters. Instead of LuxS protein, we add super folder GFP in order to make this part as the reporter part of the whole system (Fig 1). The super folder GFP is followed by the PixCell degradation tag from the 2018 Imperial College team to analyze the reporter gene spatiotemporally.


Figure 1: Quorum Sensing by Electric Stimulation

Characterization

Figure 2: Co-culture Plate under GFP (the right hole is positive anode; the left hole is the negative cathode)

Improvement Upon BBa_K2862021

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1015
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1661


[edit]
Categories
Parameters
None