Part:BBa_K3113069

DHD154b

DHD154b is a double coiled-coil structure.

Usage

To increase the modularity of our system we decided to test coiled coil proteins. These coiled coils mimic the interactions of DNA helix-helix interactions. The coiled-coil protein interaction allows the loading of more than one protein and larger proteins. We are using these helical structures to load RNA binding proteins into exosomes or virus-like-particles.

Biology

We generated helical bundle heterodimers in which each monomer is a helix–turn–helix starting from four-helix backbones produced using a generalization of the Crick coiled-coil parameterization.^[1]

Characterization

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Characterization

↑ Chen, Zibo ; Boyken, Scott E; Jia, Mengxuan ; Busch, Florian ; Flores-Solis, David ; Bick, Matthew J; Lu, Peilong ; VanAernum, Zachary L; Sahasrabuddhe, Aniruddha ; Langan, Robert A; Bermeo, Sherry ; Brunette, T J; Mulligan, Vikram Khipple ; Carter, Lauren P; DiMaio, Frank ; Sgourakis, Nikolaos G; Wysocki, Vicki H; Baker, David, Programmable design of orthogonal protein heterodimers Nature, 2018, ISSN: 1476-4687.

[1] Chen, Zibo ; Boyken, Scott E; Jia, Mengxuan ; Busch, Florian ; Flores-Solis, David ; Bick, Matthew J; Lu, Peilong ; VanAernum, Zachary L; Sahasrabuddhe, Aniruddha ; Langan, Robert A; Bermeo, Sherry ; Brunette, T J; Mulligan, Vikram Khipple ; Carter, Lauren P; DiMaio, Frank ; Sgourakis, Nikolaos G; Wysocki, Vicki H; Baker, David, Programmable design of orthogonal protein heterodimers Nature, 2018, ISSN: 1476-4687.

[1]