Part:BBa_K3002101

Level 1 � Mutant PETase + HA (Phytobrick)

This composite part contains the PAR-promoter (<a href="https://parts.igem.org/Part:BBa_K3002027">BBa_K3002027</a>) in combination with the RPL23-Terminator (<a href="https://parts.igem.org/Part:BBa_K3002006">BBa_K3002006</a>, an HA-tag (<a href="https://parts.igem.org/Part:BBa_K3002017">BBa_K3002017</a>)and the coding sequence of the mutant PETase, Mutate M, of the iGEM team TJUSLS China (<a href="https://parts.igem.org/Part:BBa_K1921003">BBa_K1921003</a>)plus the MoClo connectors for positions B2-B3 ( <a href="https://parts.igem.org/Part:BBa_K3002303">BBa_K3002303</a>), B4-B5 ( <a href="https://parts.igem.org/Part:BBa_K3002304">BBa_K3002304</a>) and B5-B6 (<a href="https://parts.igem.org/Part:BBa_K3002305">BBa_K3002305</a>).

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 249
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 1722
  
• 1000
  COMPATIBLE WITH RFC[1000]