Composite

Part:BBa_K3099002

Designed by: Heyu Gao   Group: iGEM19_USTC   (2019-10-15)
Revision as of 11:57, 20 October 2019 by Gaoheyu (Talk | contribs)


PLac+omcA+Mtr

Mtr CAB is a protein complex located on the outer membrane of Shewanella, transferring electrons from the cityplasm to the outside of the bacteria. Mtr CAB consists of three proteins, Mtr A, Mtr B, Mtr C. Mtr B is anchored onto the outer membrane. With its β barrel conformation, it can help to locate the Mtr A and Mtr C and increase the whole complex’s stability. Mtr A and Mtr C are the two protein that can actually transfer electrons with the heme attached into these two protein at the right position. MtrA is a 32-kD periplasmic decaheme cytochrome c, and MtrC is a 69-kD cell-surface-exposed. We improve the existing parts by changing the promoter and make the parts can express in Shewanella Oneidensis MR-1

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 2322
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 384
    Illegal NgoMIV site found at 410
    Illegal NgoMIV site found at 1095
    Illegal NgoMIV site found at 4523
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2709


[edit]
Categories
Parameters
None