Coding

Part:BBa_K654058

Designed by: Cody Tramp   Group: iGEM11_Utah_State   (2011-08-12)
Revision as of 00:26, 29 September 2011 by Catramp (Talk | contribs)

Thioesterase (TesA from E. coli) with 8-His Tag

TesA thioesterase gene from E. coli. Contains a 8-Histidine tag immediately before the stop codon to allow gel identification and extraction. The TesA thioesterase converts fatty acyl-ACP into fatty acids with a hydration reaction.


2011 Utah State Thioesterase.png


Thioesterase is used in conjunction with Fatty Acyl-CoA Synthetase to greatly increase the concentration of Fatty Acyl-CoA present in the cell, to increase the amount of bioproducts produced. It is used to produce Fatty Alcohols and Wax Esters in the 2011 Utah_State iGEM project.


2011 Utah State Pathway 2.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None