Part:BBa_K3112042
Chromosomal stabilization element group example 1
Our chromosome stabilizing elements consist of two parts: centromeres and tag for screening.
In this example, we try to directly replace the centromere of Saccharomyces cerevisiae.
Before chromosome transferring, we hoped the element group will not have a great impact on the growth and division of cells. It can stabilize this chromosome with stable elements when the other organism of the fusion system is dominant.
In order to meet the relevant iGEM standards for part, we deleted the homologous arms of Saccharomyces cerevisiae on both sides of the part. That doesn't mean we did the same in the experiment. The significance of creating this example is that we hope that later teams can add the stable element of chromosome that can affect cell growth to any position through PCR or other means.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 906
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 797
Illegal BglII site found at 857 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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