Coding

Part:BBa_K3117020

Designed by: Lena Schorr   Group: iGEM19_FAU_Erlangen   (2019-10-13)
Revision as of 22:34, 21 October 2019 by Lena Schorr (Talk | contribs)


scFv VH against CD3 codon optimized for CHO expression

Part BBa_K3117020 is a single chain variable fragment (scFv) of the anti-CD3 antibody. This fragment was used to generate a fusion protein to create a bispecific antibody on the one hand driected against CD3, on the other part against GPA33.



Usage and Biology

CD3 is co-expressed with the T cell receptor on T cells. Binding leads to downstream signaling cascades and activation of the T cell[2].

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 142
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None